VIOCHEM URIC ACID
Application  For the determination of Uric Acid in Serum or Plasma
Method Uricase/ PAP
Principle
 
Uricase/ PAP
 
Uric Acid + H2O
Allantoin + H2O2
 
Peroxidase
H2O2 + 4 Aminoantipyrine + Phenolic Compound
Red Quinoneimine Dye +H2O
Reagent System Two reagent, Mix Ratio 4 + 1
Storage/ Stability 2-8°C 18 Months
In Use Stability Unmixed 2-8°C 18 Months
  Mixed 2-8°C 4 Weeks
Test Parameters
Reaction End point Interval -
Wavelength 520 nm Sample Vol. 0.02 ml
Zero Settings Reagent blank Reagent Vol. 1.00 ml
Incub. Temp. 37°C / R.T. Standard 8 mg/ dl

Incub. Time

5 min. / 15 min. Factor -
Delay Time - React. Slope Increasing
Read Time - Linearity 20 mg/ dl
No. of read. - Units mg/ dl

Presentation
Pack
Cat. No.
VIOCHEM URIC ACID
25 ml
705120025