VIOCHEM UREA GLDH
Application  For the determination of Urea in Serum or Plasma
Method GLDH (U.V. Kinetic)
Principle
 
Urease
 
Urea + H2O + 2H+ 
2 NH4+ + CO2
GLDH
2 NH4+  + 2aKG  + 2NADH
2 L- Glutamate +2 NAD+  + 2H2O
Reagent System Two reagent, Mix Ratio 4 +1
Storage/ Stability 2-8°C 18 Months
In Use Stability  Unmixed 2-8°C 18 Months
Mixed 2-8°C 10 Days
Test Parameters
Reaction Fixed Time Kinetic Interval 60 sec.
Wavelength 340 nm Sample Vol. 0.01 ml
Zero Settings Distilled Water Reagent Vol. 1.00 ml
Incub. Temp. 30°C / 37°C Standard 40 mg/ dl

Incub. Time

-

Factor -
Delay Time 30 sec. React. Slope Decreasing
Read Time 60 sec. Linearity 250 mg/ dl
No. of read. 2 Units mg/ dl

Presentation
Pack
Cat. No.
VIOCHEM UREA GLDH
25 ml
705030025