VIOCHEM UREA BERTHELOT
Application  For the determination of Urea in Serum, Plasma and Urine
Method Modified Berthelot
Principle
 
Urease
 
Urea + H2
Ammonia + CO2
Alkaline
Ammonia + Phenolic Chromogen + Hypochlorite
Green Coloured Complex
 
Medium
Reagent System Three reagents,
Mix Ratio 10+1(L1+L2)
Storage/ Stability 2-8°C 18 Months
In Use Stability  Unmixed 2-8°C 18 Months
Mixed 2-8°C 4 Weeks
Test Parameters
Reaction End point Interval -
Wavelength 570 nm Sample Vol. 0.01 ml
Zero Settings Reagent blank Reagent Vol. 1.30 ml
Incub. Temp. 37°C/ R.T. Standard 40 mg/ dl

Incub. Time

5 min. + 5 min.
or
10 min. + 10 min.

Factor -
Delay Time - React. Slope Increasing
Read Time - Linearity 250 mg/ dl
No. of read. - Units mg/ dl

Presentation
Pack
Cat. No.
VIOCHEM UREA BERTHELOT
120T
705170120