VIOCHEM SGPT (ALAT)
Application  For the determination of SGPT (ALAT) activity in Serum
Method Modified IFCC (U.V. Kinetic)
Principle
 
SGPT
 

L-Alanine + α Ketoglutarate

Pyruvate + L-Glutamate  
 
LDH
Pyruvate + NADH + H+
Lactate + NAD+
Reagent System Two reagent, Mix Ratio 4+ 1
Storage/ Stability 2-8°C 18 Months
In Use Stability  Unmixed 2-8°C 18 Months
Mixed 2-8°C 3 Weeks
Test Parameters
Reaction U.V. Kinetic Interval 60
Wavelength 340 nm Sample Vol. 0.10 ml
Zero Settings Distilled Water Reagent Vol. 1.00 ml
Incub. Temp. 37°C Standard -

Incub. Time

- Factor 1746
Delay Time 60 sec, React. Slope Deccreasing
Read Time 180 sec. Linearity 500 U/L
No. of read. 4 Units U/L

Presentation
Pack
Cat. No.

VIOCHEM SGPT (ALAT)

25 ml
705060025