VIOCHEM CHOLESTEROL
Application  For the determination of Cholesterol in Serum
Method PAP
Principle

Cholesterol Esterase       

Cholesterol esters+ H2O
Cholesterol+ Fatty acids

Cholesterol Oxidase       

      
Cholesterol + O2
Cholestenone + H2O2
Peroxidase       
H2O2 + 4 Aminoantipyrine + Phenol  
Red Quinoneimine dye  + H2O
Reagent System Single reagent and a standard
Storage/ Stability 2-8°C 18 Months
In Use Stability  2- 8°C 18 Months
Test Parameters
Reaction - Interval -
Wavelength 505 nm (Hq 546nm) Green Sample Vol. 0.01 ml
Zero Settings - Reagent Vol. 1.00 ml
Incub. Temp. 37°C  / R.T. Standard 0.01 ml

Incub. Time

75-80 min. Factor -
Delay Time - React. Slope Increasing
Read Time - Linearity Upto 750 mg/dl
No. of read. - Units mg/dl

Presentation
Pack
Cat. No.
VIOCHEM CHOLESTROL
80ml (8X10ml)
705050080