VIOCHEM BILIRUBIN
Application  For the determination of Direct & Total Bilirubin in Serum
Method Modified Jendrassik & Grof’s
Principle
For Direct Bilirubin
Bilirubin +   DSA 
Azobilirubin Compound
For Total Bilirubin
Bilirubin + DSA + Caffeine Benzoate 
Azobilirubin Compound
(Pink Colour)
Reagent System Two reagents each for Total and Direct
Storage/ Stability R.T. 24 Months
In Use Stability Unmixed R.T 24 Months
Test Parameters
Reaction End point Interval -
Wavelength 546 nm Sample Vol. 0.1 ml
Zero Settings Sample Blank Reagent Vol. 1.05 ml
Incub. Temp. R.T. Standard -

Incub. Time

5 min. / 10 min. Factor 13.0
Delay Time - React. Slope Increasing
Read Time - Linearity 20 mg/dl
No. of read. - Units mg/dl

Presentation
Pack
Cat. No.
VIOCHEM BILIRUBIN
30T
705010030