VIOCHEM ALKALINE PHOSPHATASE (DEA)
Application  For the determination of Alkaline Phosphatase in Serum
Method Modified DGKC, DEA Buffer
Principle

ALP         

      p-Nitrophenyl Phosphate 

Phosphatase + p-Nitrophenol
Reagent System Two reagent, Mix Ratio 4 +1
Storage/ Stability 2-8°C 18 Months
In Use Stability Unmixed 2- 8°C 18 Months
Mixed 2- 8°C 4 Weeks
s
Test Parameters
Reaction Kinetic Interval 60 sec.
Wavelength 405 nm Sample Vol. 0.02 ml
Zero Settings Distilled Water Reagent Vol. 1.00 ml
Incub. Temp. 37°C Standard -

Incub. Time

- Factor 2754
Delay Time 60 sec. React. Slope Increasing
Read Time 180 sec. Linearity 680 U/L
No. of read. 4 Units U/L

Presentation
Pack
Cat. No.
VIOCHEM ALKALINE PHOSPHATASE 20T (20X1ml) 705100020